Does anyone have experience creating Cas9 stable cells then using sgRNA amplicons to create genomic deletions in cell lines?Was it efficient?

This method was outlined in the Zhang lab paper. I previously tried transient transfection of lenticrispr v2; which expresses both cas9 and 1 sgRNA. Actually, I took a deletion approach(2 sgRNAs...

03 June 2016 8,517 4 View