Santhosh Kalash

3 Questions 7 Answers 0 Followers

Questions related from Santhosh Kalash

How can one use DIPEA for deprotonation of primary amine groups in polyethylenimine for protein conjugation?

I wish to know how to use DIPEA or N,N-Diisopropylethylamine or Hünig's base for deprotonation of primary amine groups in polyethylenimine. Is it added based on molar ratio or weight ratio?

30 January 2015 691 2 View

Can somebody help me with primer designing for RT PCR? Which online software can I rely upon?

Are the parameters same as that for normal PCR? Please explain in detail.

22 September 2012 6,247 17 View

How to create a heatmap using circos software?

Can anyone tell me how to create a heatmap using circos software? I also wish to know the input format to be fed in the software. Anyone who has the knowledge on Perl can really help me in...

07 September 2012 3,080 6 View