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I have currently knocked out the gene to see the effect, but how can I characterize it better? I am working on two genes of 1.5-2 kbp and thir function is currently described as "miscRNA" without...
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So, I am trying to amplify a 1000 bp sequence from genomic DNA. I've amplified 5 other regions of the same genomic DNA, also on 1000 bp, which have worked perfectly. What happens is:I make a...
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