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I am trying to purify a GST tagged protein of 70kda ( with GST tag 96kda) expressed in Rosetta. The pI of protein is 5.2. I am using the GSTrap column and AKTA prime for purification. The lysis...
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I am trying to crystallize a protein which has a hydrophobic pocket in it. The problem with the protein is that some of it precipitates while concentrating the protein in amicon concentrator....
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