Generated IRES-GFP mRNA by in vitro transcription fromT7 promotor but no GFP signal in mRNA transfected cell ?

Apart from other necessary features, my vector has followiing features in exactly same order T7 promotor - EMCV IRES- MCS - 30nucleotide poly A tail - T7 terminator. i inserted...

05 May 2018 350 11 View