Hi I'm trying look at Mre11 foci in mammalian cells after camptothecin treatment, which I was not successful at. could anyone share their experience?

the cells were treated with 25uM of camptothecin for 3h then washed and fixed with 4% formaldehyde for 10min. Permeabilization was done with 90% methanol for 5 min @ RT. Blocking was done for an...

21 November 2018 7,539 2 View