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Questions related from Sanjaya Mani Dixit
I was doing a phosphorylation study of Tau using tau-1 antibody from Millipore, I am having trouble getting bands... on contacting the company they asked me to change the blocking solution to the...
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Hello everyone, I have started doing western blot for some months but then with the new antibody from Santacruz for Bcl-2 I got lots of ghost bands.... I have tried reducing d concentration of...
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Hello Everyone, I once tried performing the DNA ladder test as a measure for apoptosis. I isolated the DNA using a kit and also quantified it, it was ok back then even purity seemed ok. However...
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Hello everyone, I just started with the flow cytometry and since I was doing it for the first time, I planned to move on with single PI staining for detection of dead cells-either late apoptosis...
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I recently tried using NAC on some cell line and wanted to check the viability of cells using MTT. I found that as soon as i add MTT to wells containing NAC it turns bluish black instantly...
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Hello everyone, I recently started culturing the cells and that since i am new into sterile working and all these, i just wanted to practice a few basics of cell culture and started in absence of...
20 December 2009 1,190 3 View