Sanjaya Mani Dixit

6 Questions 21 Answers 0 Followers

Questions related from Sanjaya Mani Dixit

Phosphorylation studies-how effective is using animal serum for blocking? TBST & PBST any significant difference?

I was doing a phosphorylation study of Tau using tau-1 antibody from Millipore, I am having trouble getting bands... on contacting the company they asked me to change the blocking solution to the...

03 March 2011 6,345 5 View

Western Blot-Bcl-2 Total Santa Cruz polyclonal antibody- ghost bands numerous

Hello everyone, I have started doing western blot for some months but then with the new antibody from Santacruz for Bcl-2 I got lots of ghost bands.... I have tried reducing d concentration of...

03 March 2011 2,235 5 View

DNA ladder apoptosis-no ladder formation

Hello Everyone, I once tried performing the DNA ladder test as a measure for apoptosis. I isolated the DNA using a kit and also quantified it, it was ok back then even purity seemed ok. However...

08 February 2011 3,527 18 View

Needed some help with flow cytometry

Hello everyone, I just started with the flow cytometry and since I was doing it for the first time, I planned to move on with single PI staining for detection of dead cells-either late apoptosis...

12 November 2010 5,546 17 View

N Acetyl cysteine and MTT--incompatible or am I only having problems

I recently tried using NAC on some cell line and wanted to check the viability of cells using MTT. I found that as soon as i add MTT to wells containing NAC it turns bluish black instantly...

27 October 2010 7,779 4 View

Culturing cell in absence of any growth factors

Hello everyone, I recently started culturing the cells and that since i am new into sterile working and all these, i just wanted to practice a few basics of cell culture and started in absence of...

20 December 2009 1,193 3 View