I am using TG1 strain of E. coli and KM13 helper phage. I am trying to propagate KM13 but I am not getting plaques. can anyone suggest me ?

I am infecting TG1 strain of E. coli with KM13 helper phage, then adding it to H-top agar and then pouring this on TYE agar plate. The incubation is at 37 degree Celsius for overnight but I am...

04 April 2019 535 11 View