Sanjai kumar Pillala

5 Questions 9 Answers 0 Followers

Questions related from Sanjai kumar Pillala

What will happen if i use dream taq polymerase instead of taq polymerase im my PCr reaction?

Taq polymerase i used was 1u/uL, Dream taq was 5u/uL. I wanted 1.25u so i took 0.25uL of Dream taq and my pcr product showed smeared images.

13 December 2016 7,590 15 View

How to extract DNA coated on whatman FTA classic cards?

I tried all the methods available on internet be it methanol extraction or using Qiagen kit. I also tried incubating FTA card pieces in TE buffer overnight at 56 C but i got nothing. Each spot is...

01 December 2016 5,286 5 View

Can RAW 264.7 cells remain constantly activated in culture due to low amount of endotoxins present in diluted 10X PBS with autoclaved distilled water?

Without any activation, I constantly observe activated RAW 264.7 cells in culture confirmed through cytokine assay of TNF. Even after a number of passages (Passage number 25 to 40) are given, TNF...

08 March 2016 3,321 4 View

Can anyone help me to find trpv knockout mice or trpv knockout cell line available?

Especially in India

24 May 2015 2,633 1 View

Can vaccines be targeted towards non-structural proteins and how effective it would be in comparison to structural proteins?

whether structural proteins are good for vaccine development or non-structural proteins?

23 January 2015 5,387 5 View