Sangeeta Kumari

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Questions related from Sangeeta Kumari

Can anyone help me with this problem of generating stable cell lines after puromycin selection?

After standardizing the optimized puromycin concentration (concentration at which nearly all cells are dead between 5-7 day), 48 hrs post transfection antibiotic was added to the cells at three...

17 April 2014 9,671 7 View

What is the best way to deal with bone sample to see the reporter gene as well staining for immuno fluorescence?

When fixating bone to observe the expression of GFP from GFP expressing reporter mouse it inhibits staining for fluorescence microscopy. We can observe the GFP in PFA fixed bone samples but then...

11 February 2014 6,573 5 View