Sangeet Lal

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Questions related from Sangeet Lal

Have you experienced a shift in the molecular weight of a protein in the elution fraction after co-immunoprecipitation when run on SDS-PAGE?

After performing the co-IP, repeatedly I see that the protein in the final elution fraction run at different molecular weight (slightly lower for the co-precipitated and higher for the target...

02 August 2013 4,822 3 View

Why do lentiviral shRNA (both not-target cotrol as well as gene-specific) transfected adherent cells become suspension after thawing from Cryo?

Melanoma cells transfected with lentiviral shRNAs, which were nicely adherent before freezing down, have transformed into suspension cells with reduced viability after thawing. Even non-target...

08 October 2012 2,098 4 View