Sang-Kyu Park

2 Questions 1 Answers 0 Followers

Questions related from Sang-Kyu Park

My gene is detected in Western blot but not detected in RT-PCR?

Hello I am working on a gene to detect in various cell lines. The gene was detected by Western blot and siRNA was also worked fine (The gene was silenced by specific siRNA transfection), but not...

22 September 2017 9,133 4 View

Can someone suggest why culturing HUVEC with 0.5% serum and without ECGS caused a lot of cell death?

I am trying to treat some compound to see gene regulation signaling in HUVEC. For that I starved HUVEC cells with phenol red free and 0.5% serum and without ECGS media for 1 day. However cells...

24 September 2015 5,308 3 View