Sandu Andrei

4 Questions 2 Answers 0 Followers

Questions related from Sandu Andrei

PBeloBAC1 digestion with HindIII , not sure it worked ?

Hello , So i digested pBeloBAC11 using HindIII ( left the eppendorfs 1 hour in the water bath for the digestion process ). After that the sample was frozen at -20 C and next day a gel...

11 November 2019 1,120 3 View

PBeloBAC1 digestion with HindIII , not sure it worked ?

Hello , So i digested pBeloBAC11 using HindIII ( left the eppendorfs 1 hour in the water bath for the digestion process ). After that the sample was frozen at -20 C and next day a gel...

05 November 2019 5,138 2 View

I can't understand the bands from Dh5 alpha cells ligation and transformation . Can someone explain ?

I m using dh5 alpha cells to insert some specific segments from influenza A . I ligated the cells using TA cloning kit and transform them . I cultivated the cells on an LB media with ampicilin ,...

05 June 2019 8,606 10 View

PCR re run?

Hello, I was doing a PCR for influenza viruses ( more specifically defective interfering segments) and i had good results beside 2 segments. I store them at -20 degrees and i was thinking to run...

05 May 2019 6,758 4 View