Does anyone use PHA-L (merck or sigma?) to stimulate INF-y and RANTES release from PBMCs ? Which conditions? How do you measure the cytokine release?

We want to use PHA-L as a positive control in PBMCs since LPS and ConA are not stimulating the release of IFN-y and RANTES. Does anyone use a similar approach?

01 February 2019 4,254 2 View