Sandra Cortes

4 Questions 3 Answers 0 Followers

Questions related from Sandra Cortes

The higher the concentration of analyte in the sample, the lower the signal intensity of the control line?

I'm developing a lateral flow immunoassay in competitive format, and once I printed and built the complete strip I test it with different concentrations of the analyte (resuspended in acetate...

17 June 2022 1,612 0 View

Suspension cells growing as adherent???

I'm working with BJAB lymphocytic cell line, culturing them with RPMI media (with L-glutamine) + 10%FBS + 1% antibiotics. After two days of changing media by centrifugation, they started to adhere...

09 November 2021 1,037 3 View

How to remove false positive from lateral flow assay?

We prepare the gold conjugate with 20 mm gold nanoparticles and 100 mM phosphate buffer pH 8.35, and then we add the mAb previously dialyzed with to a concentration of 16 ug/ml. The conjugate is...

01 July 2021 3,044 4 View

Can I use an antibody with application for FC, to do IP or WB??

Hi there! I need to do immunoprecipitation and Western blot to obtain and detect a protein and I already have conjugated antibodies with FITC and PE for flow cytometry. I was wondering if there...

18 February 2020 353 3 View