Sandip Kumar Mukherjee

3 Questions 3 Answers 0 Followers

Questions related from Sandip Kumar Mukherjee

How can i clone 17 kb e.coli plasmid for sequencing?

I'm trying to clone a E.coli 17 kb plasmid DNA for sequencing. I have contaced differenr company but their price is too high more than 55k.CAN anybody help me 1)how can i clone and sequence...

03 March 2018 3,949 10 View

I want to sequence ~17kb bacterial plasmid DNA.I have contacted various company but they provide NGS but I want Sanger.can anybody help?

If possible can I collaborate to someone.

11 November 2017 6,411 0 View

TOPO TA Cloning problem!!

I'm confused by my cloning PCR results.I have used the TOPO TA Cloning kit. I got colonies after blue-white screening. I grew them in the LB liquid culture in presence of ampicillin drug and then...

06 June 2017 1,641 2 View