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Hi everyone I am getting a visible pellet of exosome. I added 100 ul PBS to dissolve it.after pipetting it was fully dissolved but after next day I found pellets in the bottom of 1.5 ml tube. Any...
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I'm trying to clone a E.coli 17 kb plasmid DNA for sequencing. I have contaced differenr company but their price is too high more than 55k.CAN anybody help me 1)how can i clone and sequence...
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I'm confused by my cloning PCR results.I have used the TOPO TA Cloning kit. I got colonies after blue-white screening. I grew them in the LB liquid culture in presence of ampicillin drug and then...
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