Sandesh Sharma Pandit

3 Questions 6 Answers 0 Followers

Questions related from Sandesh Sharma Pandit

How do you determine what the compound actually is based on it's m/z ratio?

I did a metabolomic study, where some of my compounds are novel and have high production in one of my mutants. I have its m/z ratio. Can I identify this compound solely based on this ratio? I...

07 October 2022 8,230 4 View

I want to know how to search for promoter of any genes? Specifically I want the full promoter sequence of Aspergillus Nidulans alcA promoter?

I am planning to make an overexpression plasmid with alcA promoter and trpC terminater. Can anyone provide me the sequence of alcA promoter or even a plasmid map of such expression vector that you...

14 February 2017 921 3 View

Why does Agar persists in RNA Extraction from Top-Agar medium in fungi?

I need to extract RNA mycelium of fungi from top agar medium, using Trizol or PIG B reagent. Everything is fine except in the final step, i still get the agar persisting in my RNA sample. The agar...

29 December 2015 6,494 4 View