Is GAPDH a suitable reference gene for qPCR in transfected cells?

I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...

12 July 2020 7,253 11 View