Sana Tehreem

4 Questions 4 Answers 0 Followers

Questions related from Sana Tehreem

When can E.coli secrete most of the extracelluar protein?

Hello, I'm doing optimization of an extracellular protein cloned in the pET26b vector along with PelB leader sequence. I want to know the best time at which cells can secrete most of the protein...

14 October 2019 6,011 6 View

How to derivate compounds containing amino groups in aqueous solution??

I want to use gas chromatography to analyze substances containing amino group, but because of the strong polarity of amino group, it may damage the chromatographic column, so i need to derivate it...

27 March 2019 9,458 6 View

How to confidently predict the 3D structure of a protein?

I have tried to predicted the 3D structure by using multiple online softwares like I-TASSAR, MUSTER, Swiss-Model and (PS)2 but predicted 3D structure from each software has bad quality and can not...

28 November 2018 9,155 3 View

How to do molecular dynamics simulations for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio??

Hello, I want to do simulations analysis for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio. Kindly suggest me a protocol. Thanks

12 February 2018 9,673 3 View