I am working with primary rat neonatal fibroblasts to study cardiac fibrosis, on adding AngII (1uM) the cells die, it shouldn't be toxic. Any ideas?

The cells are transfected first with siRNA, and after 48 hours, I add AngII. After transfection, the confluency goes down, and AngII makes it even worse.

10 June 2025 4,498 2 View