Sana Mahmood

3 Questions 1 Answers 0 Followers

Questions related from Sana Mahmood

What is the acceptable coefficient of variance or SD in the CT values of reference gene in biological replicates (in treated and non treated samples)?

what is the threshold values for selecting or rejecting the gene as reference gene? how much intergroup variability can be tolerated?

01 November 2022 3,677 1 View

In bio-rad CFX manager software for qPCR, what is meant by CV and M value for Homogenous and heterogenous samples?

For my treated and non treated samples there is some difference for ct values of reference genes. I want to use two reference genes for my analysis. The target stability tab in the Bio-Rad cfx...

01 November 2022 9,509 0 View

What is the best concentration to start for making drug resistant cancer cell line: High or Low?

Should I start with IC75 or IC90 concentration to get rid of all the sensitive cells at the initial stage and then gradually work on the increasing concentration from Ic10 until IC90 to get a new...

07 April 2022 9,987 2 View