Samuel John Prendergast

3 Questions 7 Answers 0 Followers

Questions related from Samuel John Prendergast

Why is my Masson trichrome stain fading?

I am using the TCS-Bioscience kit for Masson trichrome stain of various tissues. I achieve good haematoxilin stain and red Ponceau Fuchsin for muscle tissue yet the light green Masson stain for...

08 August 2017 7,884 6 View

Any experience in TEM (Transmission Electron Microscopy) of exosomes? - What are the non-exosomal structures in my image!?

Performed TEM on exosome samples isolated from cancer cell line by UC and resuspended in PBS. the majority of the image showed strange (non-exosomal) structures that could be throwing off my NTA...

09 September 2016 3,911 5 View

Why are the CD63 western-blot bands so variable between samples/ antibodies?

According to the literature there are three transcript variants at ~17, ~23 and ~26 kDa yet my most prominent bands from HepG2 whole cell lysates (suggested by manufacturer: Abcam for 26 kDa band)...

01 January 2015 1,054 3 View