Samuel Danby

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Questions related from Samuel Danby

Is the RESTQ-Coaches questionnaire available for free download?

Looking to obtain a copy of the RESTQ-coaches questionnaire for a paper. Is it possible to get a copy, seem to only be getting data and secondary use of the questionnaire

04 April 2018 2,752 0 View

Staining OCT-1 for ICC, having non-specific binding of secondary antibody. Has anyone attempted this and had issues also?

I am doing imaging of OCT-1, and although my secondary antibody is correct, I am getting non specific binding, showing in negative control (no primary AB). Would this be related more to my 2AB or...

10 March 2018 5,089 3 View

Staining OCT-1 for ICC, having non-specific binding of secondary antibody. Has anyone attempted this and had issues also?

I am doing imaging of OCT-1, and although my secondary antibody is correct, I am getting non specific binding, showing in negative control (no primary AB). Would this be related more to my 2AB or...

03 March 2018 6,030 3 View

Best seeding practice for 96 well plates when analysing fluorescence and absorbance with alamar blue?

I am using alamar blue to test the fluroescene and absorbance on two colorectal cancer cells lines. Research/ literature I have read give different advice regarding how to seed the wells in the...

11 November 2017 8,252 1 View

Cell growth; changing growth medium and how it effects cell growth, has anyone found that the growth of the cells change when changing medium?

I have recently changed growth medium from BioWest to Corning. Has anyone encounter changes in growth to cells when changing company, found that a particular brand of medium doesn't work on some...

10 October 2017 2,265 0 View