Samu John

3 Questions 3 Answers 0 Followers

Questions related from Samu John

Why am i not getting ETS2 bands in western blot?

I have not been getting ETS2 bands with multiple antibodies (sc37354, CST 46676) although i have been getting confocal images. I have modified concentrations of gel, primary, secondary antibody...

27 October 2018 4,975 2 View

Why am I not getting MUC1 band in western blot whereas immunohistochemistry staining is working ?

MUC1 staining (DAB) has been successful for IHC, both with tris and citrate buffers, however, western blot have failed miserably. CST VU4H5 antibody is compatible for both IHC and WB. Is...

12 February 2018 7,356 10 View

Why do my SDS-PAGE bands merge (25, 37 and 50 KDa) on further running instead of resolving clearly ?

Different conc. of 30% polyacrylamide I have used but the results are the same (except for 15%gel). The dye front forms at the level of this merged band implying that any protein less than 50 KDa...

05 February 2018 8,903 2 View