Samriddhi Ghimire

2 Questions 5 Answers 0 Followers

Questions related from Samriddhi Ghimire

How to prevent non-specific signals on lateral flow assays?

I used sandwich assay on a test strip. When I allowed only PBS to flow, it still gave signals on nitrocellulose membrane where I immobilized the antibody. I tried to use a blocking agent (BSA and...

30 July 2013 9,890 2 View

Gold nanoparticle conjugated with protein A gets stuck to the wall of e-tube after centrifuging the second time.

When centrifuged the first time all GNP conjugate settles down with no problem but after removal of supernatant, addition of PBS pH 7.4 and centrifuging the second time, most of the gold...

27 June 2013 7,127 4 View