Sampurna Guha

3 Questions 2 Answers 0 Followers

Questions related from Sampurna Guha

Why I am not getting smear bands after DNA shearing of the crosslinked cell lysate using Diagenode bioruptor?

For the ChIRP MS experiment, I am sonicating the crosslinked HT29 cell lysate using diagenode bioruptor. The cell lysis buffer has 50mM tris-Cl (pH7.0), 10mM EDTA (pH8.0), 1% SDS, 1mM PMSF, 1X...

15 May 2025 9,887 0 View

Why am I unable to get transformed colonies post KLD assay with my PCR product?

Though my PCR is successful with DNA Phusion polymerase, I am still unable to get transformed colonies post KLD assay with my DH5alpha comp cells. What can be the possible reason for this? I am...

27 April 2025 2,170 1 View

Will 1X TBST affects protein purification performed using Ni-NTA column?

I unmindfully added 1X TBST in place of washing buffer to the Ni-NTA column after the flowthrough during protein purification. Though I didn't allow the beads to incubate with the buffer, I...

07 January 2025 9,467 3 View