Sampreeti Tahbildar

2 Questions 3 Answers 0 Followers

Questions related from Sampreeti Tahbildar

Why is my negative control in PCR showing smear in gel electrophoresis?

I have used the same primers for amplification and have been fine, but suddenly I started getting smear in both my negative control and test. I have checked all my reagents and NFW by using the...

28 June 2024 7,643 5 View

If a primer dimer is formed at the 5' end with a Delta G of -9.96 kcal/mol (6basepairs), is it going to affect the PCR reaction?

I have used IDT Oligoanalyzer for checking my primers. In here, the accepted delta G value for internal self‐dimers(-9kcal/mol) and the 3' end (-6kcal/mol) has been mentioned but not for the 5'end...

19 June 2023 3,562 1 View