If a primer dimer is formed at the 5' end with a Delta G of -9.96 kcal/mol (6basepairs), is it going to affect the PCR reaction?

I have used IDT Oligoanalyzer for checking my primers. In here, the accepted delta G value for internal self‐dimers(-9kcal/mol) and the 3' end (-6kcal/mol) has been mentioned but not for the 5'end...

19 June 2023 3,544 1 View