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I am trying to induce toxoplasma cyst formation (both RH and ME49) strains by altering the pH to 8.1 (DMEM, 1%FCS, 1% pen-strep), at 37oC, without CO2. However, the host cells weren't healthy and...
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I am new to Toxoplasma gondii (RH strain) work and is now optimizing the parasite number. What is the recommended host to parasite ratio? In my work, I seeded 80,000 HS68 onto a 24 well plate, let...
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