I'm having problem with transforming EcoRI linearized pRS413 plasmid into S. cerevisiae. Not getting colony on plate. Have any idea how to solve this?

I'm actually trying to transform two plasmids into yeast. 1. pRS416 with URA3 gene, 2.pRS413 with His3 gene. At first, i have transformed pRS416 into yeast that worked (got colonies on -URA...

05 May 2018 9,041 4 View