Samira Abdi

5 Questions 6 Answers 0 Followers

Questions related from Samira Abdi

Total Protein Extraction protocol?

im looking for a protocol which is specified for the extraction of whole protein (membrane, cytoplasm and nucleus). Can anyone help me wıth a rutin protocol on this??

10 October 2017 8,069 4 View

Sodium bicarbonate vs. Sodium pyruvate usage in cell culture?

Question is, why there is specifically written Sodium puruvate or Sodium bicarbonate on cell culture medium separately? are they the same? Since the role of each is difference from each other why...

02 February 2017 7,866 0 View

Why should the Tm of Probe be higher than Primer?

It is claimed that the Tm value of Probe should be 10 degree higher than that of Primer, what is the reason of this?

11 November 2016 5,246 3 View

Can anyone suggest me a reliable software to deign primer?

Hey, im trying to design primer for my genes which im going to study on... im using "PerPrimer", "Primer3", "Primer BLAST" and " IDT" softwares for designing and cheking as if it forms hairpin,...

11 November 2016 4,465 6 View

Does anyone have primer and probe sequences for IL-10 ?

I am looking for suitable primer and probe sequences for interleukin-10. Actually due to its inappropriate exon lengths it is very hard to find efficient ones. Even the ones i found from...

11 November 2016 1,387 2 View