Samir Sabri

5 Questions 5 Answers 0 Followers

Questions related from Samir Sabri

How to get the concentration of cas12f1 protein in the reaction?

hello, I was reading the following paragraph in materials and methods: "To evaluate the trans-cleavage activity of Cas12f1 RNP with different guide RNAs, a reaction buffer was prepared by mixing...

05 March 2025 8,137 4 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

19 July 2024 4,450 2 View

Difference of RNA concentration using acid phenol chloroform extraction method and qiagen kit?

hello, i am intersted in the cause of getting higher concentration of RNA when using the acid phenol chlorofom extraction to the qiagen kit.. i am extracing RNA from cells and i tried both method...

19 September 2023 6,527 0 View

Design of RBS using RBS calculator?

hello, I am curious about the use of RBS designer from the website: https://salislab.net/software/ how is it possible to use it for designing new RBS to overexpress protein in e.coli? thank you...

28 July 2023 6,240 2 View

Concentration of protein?

hello, I have a protein with a molecular weight of 100 kDa and the concentration is 1pmol/ul. how is possible to convert the protein concentration to ng/ul? thank you for your help.

20 July 2023 5,937 1 View