Why does my AAV titration via qPCR show same Cq value for 4 consecutive 5-fol dilutions?

Hello, Context: I am trying to purify and quantify my AAV containing my plasmid of interest. I used HekAAV cells from Takara and transfected the cells with my plasmid of interest, pRepcap and...

14 February 2024 9,583 3 View