Sami Gemal Mohammed

5 Questions 7 Answers 0 Followers

Questions related from Sami Gemal Mohammed

Measure transferrin or/and non-transferrin iron uptake in cells?

Hi everyone, I am trying to measure intracellular iron content from cultured cells using the colorimetry method (chromogen reagent), however so far unsuccessful. The absorbance readings at 540 nm...

09 September 2017 3,993 1 View

How do I generate stable transfection cell lines?

I made a stable cell line of a GFP fused gene which are resistance to hygromycin B. Cells in the first few passages were nearly 80-90% expressing GFP as analysed in the microscope. But after...

12 December 2015 5,801 5 View

Any one has experience with Accell siRNA (against primary cilia genes) from ThermoFisher?

Hi fellow researchers. Accell siRNA is claimed (by the company) to be good for use with primary cells (hard to transfect type of cells). The concentration of siRNA recommended for use is...

06 June 2015 8,830 3 View

What is the best flow chamber system to study flow and cilia function?

I want to study primary cilia mechanical function. For that I need a flow chamber system where I can apply flow to polarised cells (apical and basolateral). I hope you can guide me for the right...

08 August 2014 6,031 2 View

Any one tried siRNA on mouse kidney primary cells?

Primary cultures are so fragile and sensitive as you know; transfecting them would be so difficult. Did any one try Accell siRNA smart pool to achieve knockdown of genes on these cells?

08 August 2014 2,085 3 View