Sami Gemal Mohammed

5 Questions 8 Answers 0 Followers

Questions related from Sami Gemal Mohammed

Does anyone have experience with Ibidi flow chamber systems?

I am trying to set-up a flow chamber system to study the function of primary cilia. Lots of chambers are commercially available but none are really user friendly. Ibidi seem relatively easy to...

18 December 2015 6,459 1 View

What do you think of using Chloral hydrate to remove primary cilia from cells?

Chloral hydrate might be toxic to cells by itself. But I assume with the right concentration you can keep the viability of cells while cilia are detached. The question is do you know any...

28 August 2015 7,948 4 View

Any one tried siRNA on mouse kidney primary cells?

Primary cultures are so fragile and sensitive as you know; transfecting them would be so difficult. Did any one try Accell siRNA smart pool to achieve knockdown of genes on these cells?

22 August 2014 5,998 2 View

What is the best flow chamber system to study flow and cilia function?

I want to study primary cilia mechanical function. For that I need a flow chamber system where I can apply flow to polarised cells (apical and basolateral). I hope you can guide me for the right...

15 August 2014 8,594 2 View

Does any one know how to easily measure primary cilia length?

I use the most commonly used cilia marker, acetylated alfa tubulin, to stain primary cilia. With the help of confocal microscope I take a z-scan image of cilia. I, however, looking for a software...

15 August 2014 7,823 5 View