Sameena Parveen

2 Questions 5 Answers 0 Followers

Questions related from Sameena Parveen

Not getting Knock down either with siRNA or shRNA

Hello everyone, I am working on MDAMB231 cell line in which I have to knock down molecule of 25-37Kda .I tried both siRNA(Dharmafect mediated,Invitrogen) and shRNA(LTX,PLUS mediated,Invitrogen)...

07 July 2017 4,622 4 View

What's the mutation or changes did occur in normal breast cells that give rise to MDA-MB231 and MCF-10A?

I'm working on this cell line and know only that MDA-MB231 is TNBC, highly aggressive & metastatic while MCF10A is immortalised non cancerous cell line.But i want to know what changes lead to...

02 February 2016 1,555 3 View