I am using TCBS agar to plate my Vibrio parahaemolyticus, but my OD600 and plate counts are very inconsistent. What is wrong?

How I make my culture: take a lump from my glycerol stock of my pathogenic strain of Vibrio parahaemolyticus into a 5mL tube of TSB spiked with 2% NaCl. I subculture that after two hours into 95...

05 May 2018 5,210 2 View