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Questions related from Samantha Locke
I have homogenized rat plantar skin in lysis buffer and centrifuged at 13 500rpm for 50 minutes. I noticed that there seemed to be 2 phases in the supernatant so recentrifuged it. there are now...
04 April 2015 3,446 3 View
I have ground up rat plantar skin for western blots. I wish to probe for a neurotrophin. They look terrible, lots of spurious bands at the concentration needed to detect my protein of interest. I...
04 April 2015 6,970 5 View
I am trying to probe for a protein by western blot. I have homogeneized paw skin in lysis buffer which was then centrifuged for 50 mins at 13000 rpm. The supernatant was then taken and made into...
02 February 2015 5,604 5 View
