Samantha Locke

2 Questions 3 Answers 0 Followers

Questions related from Samantha Locke

Why does my western blot from skin homogenate look so bad?

I have ground up rat plantar skin for western blots. I wish to probe for a neurotrophin. They look terrible, lots of spurious bands at the concentration needed to detect my protein of interest. I...

24 April 2015 8,681 4 View

Why does the cell lysate supernatant have 2 phases?

I have homogenized rat plantar skin in lysis buffer and centrifuged at 13 500rpm for 50 minutes. I noticed that there seemed to be 2 phases in the supernatant so recentrifuged it. there are now...

23 April 2015 7,558 3 View