Samantha Chan

2 Questions 3 Answers 0 Followers

Questions related from Samantha Chan

Why do the results of TCID50 and qPCR show inconsistent, when detecting virus titer?

Dear all, I am studying A GENE influence viral replication and translation OR NOT. After knocking out THAT GENE by CRISPR, I infected the cells (both WT and KO) with virus at MOI of 0.02 and 0.2 ....

27 September 2024 327 4 View

Why was the protein be detected by Coomassie but not by western blot, after being purified by Ni-NTA?

Dear all, I constructed plasmids with 12x His-tag at C-terminus to express the fusion proteins (64kDa). After purified by Ni2+ column, there happened to be correct bands between 55~70kDa...

25 September 2024 3,877 4 View