Why do I not see any bands on SDS-PAGE while the Bradford colorimetric assay gives blue color ?

I am purifying a recombinant protein (MW 37 kD) using IMAC. I used the Bradford colorimetric assay (by adding a drop of the solution from the column to Coomassie blue G-250) on the wash and...

08 April 2020 3,292 9 View