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I am culturing cancer cells and noticing strange growth in the media. To me it doesn't look like bacteria or yeast, and not consistent with images I've seen online of fungi in media. I removed the...
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Hi, we are having trouble growing isolated mouse cortical astrocytes on coverslips for immmunofluorescence. We isolated the cells and expanded and froze some vials. After thawing, the astrocytes...
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We thawed and seeded a vial of bEnd.3 cells (ATCC) last week, but after 10 days they do not look like endothelial cells. The suggested media is DMEM + 10% FBS; we have been successfully growing...
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Hello, after a stress protocol in mice I have seen changes in Fgf2 gene expression in the brain and hoping now to check protein levels. Immunofluorescence would be ideal as I already have flash...
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