Sam Dundon

2 Questions 3 Answers 0 Followers

Questions related from Sam Dundon

Recommendations for a fission yeast medium + sorbitol recipe?

I need to make plates of fission yeast YE5S medium with 1.2 M sorbitol. An old protocol in the lab says to make 0.5 L of 2.4 M sorbitol as well as 0.5 L YE5S + enough agar for 1 L, autoclave...

15 August 2016 8,497 1 View

How can I reduce background binding in an RNA IP?

I am trying to pull down a protein with its RNA binding partners using antibody-coupled Dynabeads after UV-crosslinking. However, I am using IgG coupled beads as a negative control and getting...

21 April 2016 8,429 2 View