The use of RIPA lysis buffer for examining phospho-ERK(1/2) in my human dermal fibroblast cells (adult).

I was hoping to improve upon a cocktail that I currently use; RIPA lysis buffer (1x Modified RIPA lysis buffer: 10mM Tris-HCl, pH7.5; 10mM EDTA; 1% NP-40; 0.1% SDS; 150nM NaCl; 0.5% sodium...

18 February 2013 6,229 5 View