Western Blot result / interpretation band appear grey & blurry. How do I fix the problem? Western Blot used HOEFER for transferring membrane, help?

I used muscle cells as sample, and the western blot band appear grey and blurry. I used non-fat milk for blocking, does this has any effect? This is my 10th time trying and the band still appear...

07 February 2023 4,512 1 View