Salma El-Shafie

5 Questions 8 Answers 0 Followers

Questions related from Salma El-Shafie

Cells look weird instantly as soon as I replace media they're seeded in with fresh warmed media (same media seeded in) they become smaller round/oval?

I never used hepes for buffering ph and never had this problem with these cells for years. Pics are for A549 in wells (looking good before changing media) and looking rounded and bubbley once...

02 January 2018 1,673 6 View

How to achieve good dispersion of seeded cells within wells and avoid ending up with most cells in the center while the rest is almost clear?

I don't have this problem in flasks or big dishes, just in 12-well and 96-wells. After seeding i move the flask/dish in the 4 directions N,S,E,W on the bench to help disperse them but they remain...

05 December 2017 9,273 0 View

How do you prepare agarose coated slides for comet assay? (in clear details please)?

Do u use special kind of glass slides, how do you level/flatten the molten agarose, how much volume of agarose do you use, if you dip your slides how do you achieve this (they drip off the slide...

10 November 2016 8,019 8 View

Comet assay-no tails, thoughts where to start troubleshooting?

Hello! I'm still trying to get the assay to work, first time to do it. Followed the standard Singh et all 1988 protocol for alkaline comet assay. I used different drugs (0.1%H2O2 and 18ug/ml...

10 November 2016 4,357 6 View

What do I need to start/establish a comet assay experiment in the lab?

I find online kits and equipment specialised for comet assay. My question is: Can't I use a standard gel electrophoresis unit (as the pic) and prepare the buffers for (neutral DSB or alkaline SS...

16 October 2016 6,600 5 View