Salim Timo Islam

4 Questions 6 Answers 0 Followers

Questions related from Salim Timo Islam

Which ion chelators discriminate best between Ca2+, Zn2+, Mg2+, and Mn2+?

I have a proteolytic enzyme activity that is inhibited by EDTA, suggesting that the enzyme I'm investigating is a metalloprotease. Unfortunately, EDTA can chelate several divalent cations,...

13 August 2015 6,093 8 View

For a lipoprotein, what is the best way to determine the site of lipid attachment to the protein?

I have a protein that fractionates with the outer membrane sample of Gram-negative bacterial cells. SignalP4.1 predicts that the native protein is processed between amino acids...

21 January 2015 4,311 3 View

What is the best way to adjust the pH of LB broth for bacterial growth?

I would like to grow my cells in broth at different pH values. However, over time, the pH of a culture will change as the cell density increases. What would be the best way in which to maintain an...

07 March 2013 9,854 18 View

Why does the turbidity of my detergent-solubilized mixture of lipid and membrane protein change depending on the protein:lipid ratio?

Scenario: --> 5 tubes, all with the same amount of dried-down lipid (e.g. 1 mg). --> In the end, each 1 mg of lipid will be solubilized in the same final volume (e.g. 1 mL) --> in the end, each...

02 November 2012 1,928 9 View