Salila Pradhan

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Questions related from Salila Pradhan

I haver synthesized cDNA from plant RNA samples. Then I did PCR where I got nice bands. But there is no amplification in qRT-PCR using same cDNA. why?

I have synthesized cDNA using one microgram RNA template. Then, I diluted it to five times. Then, I did PCR using diluted cDNA with housekeeping gene act2. In PCR, I got nice bands in the gel....

28 September 2023 8,082 4 View

I have synthesized cDNA from plant RNA samples. Then I did PCR where I got nice bands. But there is no amplification in qRT-PCR using same cDNA. why?

I have synthesized cDNA using one microgram RNA template. Then, I diluted it to five times. Then, I did PCR using diluted cDNA with housekeeping gene act2. In PCR, I got nice bands in the gel....

28 September 2023 945 4 View

Is there any reliable free or paid software to analyse plant root structure? or is anyone analysing plant root structure using any software?

I have two sets of plants where I can see the difference in root structure. Manually it can be done, but it will be time-consuming. So, if anyone is using any software or any method to analyse...

24 August 2023 3,076 2 View