Salem Baldi

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Questions related from Salem Baldi

Any suggestion could help me design primers that cover specific nucleotides directly by forcing the primers to include them at their ends ?

I have specific nucleotide sequence( from RNA-seq) and looking for a design assay enable me design primers that cover these specific nucleotides directly by forcing the primers to include them at...

06 November 2024 7,237 5 View

Could both knockdown and over-expression affect the expression of target gene ?

We recently used siRNA to knock down a gene in the HCT116 cell line. The knockdown had no effect on target gene expression, whereas overexpression had a minor effect in the same cell line and...

11 September 2021 5,901 4 View