Sakshi Mehta

4 Questions 10 Answers 0 Followers

Questions related from Sakshi Mehta

Has anybody used mdi gel extraction kit (range upto 10KB) for PB4NU RD fragment elution?

Hi! I need help with PB4NU (14KB) restriction enzyme digested fragment elution. I cannot order a kit specially for this but I need to elute it. The problem is I tried using Qiagen and mdi both...

08 August 2018 9,509 1 View

Trouble in amplifying a particular gene?

I have trouble in amplifying a gene. I am continuously trying to find a possible solution. All methods have failed me somehow. I was amplifying gene A using gene specific primers. This gene has a...

05 May 2018 6,414 9 View

How to make sure if your RNA has been converted to cDNA?

I am trying to amplify a gene from cDNA. The gene contains an intron, therefore, I am amplifying it from cDNA. When I amplified it using genomic DNA, it shows the desired band. But when I am...

04 April 2018 2,535 9 View

How to reduce degradation in Agrobacterium RNA ?

I am isolating RNA of Agrobacterium and when I performed it for the first time it showed no degradation and now it is showing degradation. What can be the possible cause? I used Trizol method...

02 February 2018 5,610 6 View